Translational_Unit

Part:BBa_K4310015:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


quorum-sensing induced expression of eGFP in plant (2)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 12
    Illegal PstI site found at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 12
    Illegal NheI site found at 3040
    Illegal PstI site found at 938
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 12
    Illegal BglII site found at 30
    Illegal BglII site found at 58
    Illegal BglII site found at 86
    Illegal BglII site found at 114
    Illegal BglII site found at 1856
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 12
    Illegal PstI site found at 938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 12
    Illegal PstI site found at 938
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1167
    Illegal BsaI site found at 3256
    Illegal BsaI.rc site found at 188
    Illegal BsaI.rc site found at 1446
    Illegal BsaI.rc site found at 3535
    Illegal SapI site found at 202
    Illegal SapI site found at 251
    Illegal SapI site found at 2069
    Illegal SapI site found at 2118
    Illegal SapI.rc site found at 236
    Illegal SapI.rc site found at 983
    Illegal SapI.rc site found at 2103
    Illegal SapI.rc site found at 3072


Design Notes

To make compatible to our assembly, CAMV 35S and Tnos are amplified by overhang primers.


Source

Tra(4X)::m35S: BBa_K4310000 Kozak: BBa_K4310003 eGFP: BBa_K4310006 Tnos: BBa_J428082 from distribution 2022 CAMV 35S: BBa_J428074 from distribution 2022 BBa_K2516001 VP16::TraR: BBa_K4310001

References